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Clonexpress inc clonexpress® multis one step cloning kit
Clonexpress® Multis One Step Cloning Kit, supplied by Clonexpress inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The construction and structure of the Raf-Fluc. In the flow chart, the fragments were amplified by PCR from the indicated plasmids or the cDNA library <t>of</t> <t>K562</t> cell line, and seamlessly constructed into pcDNA3.1(+) using ClonExpress TM <t>MultiS</t> One Step Cloning kit. The NLuc and CLuc fragments of split firefly luciferase were respectively fused to the N- and C-terminals of Raf-1, through short flexible linkers (G 2 S) 4 or (G 4 S) 2 . The hybrid firefly luciferase (hybrid Fluc) and gaussia luciferase (Gluc) were coexpressed via the IRES under the control of the CMV promoter.
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The construction and structure of the Raf-Fluc. In the flow chart, the fragments were amplified by PCR from the indicated plasmids or the cDNA library <t>of</t> <t>K562</t> cell line, and seamlessly constructed into pcDNA3.1(+) using ClonExpress TM <t>MultiS</t> One Step Cloning kit. The NLuc and CLuc fragments of split firefly luciferase were respectively fused to the N- and C-terminals of Raf-1, through short flexible linkers (G 2 S) 4 or (G 4 S) 2 . The hybrid firefly luciferase (hybrid Fluc) and gaussia luciferase (Gluc) were coexpressed via the IRES under the control of the CMV promoter.
Gibson Assembly Clonexpress Multis One Step Cloning Kit, supplied by Clonexpress inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Clonexpress inc multis one step cloning kit c113
The construction and structure of the Raf-Fluc. In the flow chart, the fragments were amplified by PCR from the indicated plasmids or the cDNA library <t>of</t> <t>K562</t> cell line, and seamlessly constructed into pcDNA3.1(+) using ClonExpress TM <t>MultiS</t> One Step Cloning kit. The NLuc and CLuc fragments of split firefly luciferase were respectively fused to the N- and C-terminals of Raf-1, through short flexible linkers (G 2 S) 4 or (G 4 S) 2 . The hybrid firefly luciferase (hybrid Fluc) and gaussia luciferase (Gluc) were coexpressed via the IRES under the control of the CMV promoter.
Multis One Step Cloning Kit C113, supplied by Clonexpress inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CELLAGEN TECHNOLOGY LLC clonexpress ii one step cloning kit
The construction and structure of the Raf-Fluc. In the flow chart, the fragments were amplified by PCR from the indicated plasmids or the cDNA library <t>of</t> <t>K562</t> cell line, and seamlessly constructed into pcDNA3.1(+) using ClonExpress TM <t>MultiS</t> One Step Cloning kit. The NLuc and CLuc fragments of split firefly luciferase were respectively fused to the N- and C-terminals of Raf-1, through short flexible linkers (G 2 S) 4 or (G 4 S) 2 . The hybrid firefly luciferase (hybrid Fluc) and gaussia luciferase (Gluc) were coexpressed via the IRES under the control of the CMV promoter.
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Clonexpress inc multis single-step fusion cloning kit
The construction and structure of the Raf-Fluc. In the flow chart, the fragments were amplified by PCR from the indicated plasmids or the cDNA library <t>of</t> <t>K562</t> cell line, and seamlessly constructed into pcDNA3.1(+) using ClonExpress TM <t>MultiS</t> One Step Cloning kit. The NLuc and CLuc fragments of split firefly luciferase were respectively fused to the N- and C-terminals of Raf-1, through short flexible linkers (G 2 S) 4 or (G 4 S) 2 . The hybrid firefly luciferase (hybrid Fluc) and gaussia luciferase (Gluc) were coexpressed via the IRES under the control of the CMV promoter.
Multis Single Step Fusion Cloning Kit, supplied by Clonexpress inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Absource Diagnostics GmbH clonexpress multis one step cloning kit
The construction and structure of the Raf-Fluc. In the flow chart, the fragments were amplified by PCR from the indicated plasmids or the cDNA library <t>of</t> <t>K562</t> cell line, and seamlessly constructed into pcDNA3.1(+) using ClonExpress TM <t>MultiS</t> One Step Cloning kit. The NLuc and CLuc fragments of split firefly luciferase were respectively fused to the N- and C-terminals of Raf-1, through short flexible linkers (G 2 S) 4 or (G 4 S) 2 . The hybrid firefly luciferase (hybrid Fluc) and gaussia luciferase (Gluc) were coexpressed via the IRES under the control of the CMV promoter.
Clonexpress Multis One Step Cloning Kit, supplied by Absource Diagnostics GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Clonexpress inc bifc constructs clonexpress multis one step cloning kit
The construction and structure of the Raf-Fluc. In the flow chart, the fragments were amplified by PCR from the indicated plasmids or the cDNA library <t>of</t> <t>K562</t> cell line, and seamlessly constructed into pcDNA3.1(+) using ClonExpress TM <t>MultiS</t> One Step Cloning kit. The NLuc and CLuc fragments of split firefly luciferase were respectively fused to the N- and C-terminals of Raf-1, through short flexible linkers (G 2 S) 4 or (G 4 S) 2 . The hybrid firefly luciferase (hybrid Fluc) and gaussia luciferase (Gluc) were coexpressed via the IRES under the control of the CMV promoter.
Bifc Constructs Clonexpress Multis One Step Cloning Kit, supplied by Clonexpress inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Clonexpress inc linearized aav plasmids clonexpress multis one step cloning kit
The construction and structure of the Raf-Fluc. In the flow chart, the fragments were amplified by PCR from the indicated plasmids or the cDNA library <t>of</t> <t>K562</t> cell line, and seamlessly constructed into pcDNA3.1(+) using ClonExpress TM <t>MultiS</t> One Step Cloning kit. The NLuc and CLuc fragments of split firefly luciferase were respectively fused to the N- and C-terminals of Raf-1, through short flexible linkers (G 2 S) 4 or (G 4 S) 2 . The hybrid firefly luciferase (hybrid Fluc) and gaussia luciferase (Gluc) were coexpressed via the IRES under the control of the CMV promoter.
Linearized Aav Plasmids Clonexpress Multis One Step Cloning Kit, supplied by Clonexpress inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vazyme Biotech Co clonexpress ii one step cloning kit
The construction and structure of the Raf-Fluc. In the flow chart, the fragments were amplified by PCR from the indicated plasmids or the cDNA library <t>of</t> <t>K562</t> cell line, and seamlessly constructed into pcDNA3.1(+) using ClonExpress TM <t>MultiS</t> One Step Cloning kit. The NLuc and CLuc fragments of split firefly luciferase were respectively fused to the N- and C-terminals of Raf-1, through short flexible linkers (G 2 S) 4 or (G 4 S) 2 . The hybrid firefly luciferase (hybrid Fluc) and gaussia luciferase (Gluc) were coexpressed via the IRES under the control of the CMV promoter.
Clonexpress Ii One Step Cloning Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The construction and structure of the Raf-Fluc. In the flow chart, the fragments were amplified by PCR from the indicated plasmids or the cDNA library of K562 cell line, and seamlessly constructed into pcDNA3.1(+) using ClonExpress TM MultiS One Step Cloning kit. The NLuc and CLuc fragments of split firefly luciferase were respectively fused to the N- and C-terminals of Raf-1, through short flexible linkers (G 2 S) 4 or (G 4 S) 2 . The hybrid firefly luciferase (hybrid Fluc) and gaussia luciferase (Gluc) were coexpressed via the IRES under the control of the CMV promoter.

Journal: Scientific Reports

Article Title: Noninvasive Imaging of Ras Activity by Monomolecular Biosensor Based on Split-Luciferase Complementary Assay

doi: 10.1038/s41598-017-08358-3

Figure Lengend Snippet: The construction and structure of the Raf-Fluc. In the flow chart, the fragments were amplified by PCR from the indicated plasmids or the cDNA library of K562 cell line, and seamlessly constructed into pcDNA3.1(+) using ClonExpress TM MultiS One Step Cloning kit. The NLuc and CLuc fragments of split firefly luciferase were respectively fused to the N- and C-terminals of Raf-1, through short flexible linkers (G 2 S) 4 or (G 4 S) 2 . The hybrid firefly luciferase (hybrid Fluc) and gaussia luciferase (Gluc) were coexpressed via the IRES under the control of the CMV promoter.

Article Snippet: In the flow chart, the fragments were amplified by PCR from the indicated plasmids or the cDNA library of K562 cell line, and seamlessly constructed into pcDNA3.1(+) using ClonExpress TM MultiS One Step Cloning kit.

Techniques: Amplification, cDNA Library Assay, Construct, Cloning, Luciferase, Control