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Image Search Results
Journal: Scientific Reports
Article Title: Noninvasive Imaging of Ras Activity by Monomolecular Biosensor Based on Split-Luciferase Complementary Assay
doi: 10.1038/s41598-017-08358-3
Figure Lengend Snippet: The construction and structure of the Raf-Fluc. In the flow chart, the fragments were amplified by PCR from the indicated plasmids or the cDNA library of K562 cell line, and seamlessly constructed into pcDNA3.1(+) using ClonExpress TM MultiS One Step Cloning kit. The NLuc and CLuc fragments of split firefly luciferase were respectively fused to the N- and C-terminals of Raf-1, through short flexible linkers (G 2 S) 4 or (G 4 S) 2 . The hybrid firefly luciferase (hybrid Fluc) and gaussia luciferase (Gluc) were coexpressed via the IRES under the control of the CMV promoter.
Article Snippet: In the flow chart, the fragments were amplified by PCR from the indicated plasmids or the cDNA library of K562 cell line, and seamlessly constructed into pcDNA3.1(+) using
Techniques: Amplification, cDNA Library Assay, Construct, Cloning, Luciferase, Control